vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2025-22-2-119-133vestim-1029Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEСравнительный анализ эффективности остеогенеза в периимплантатном дефекте при использовании скаффолдов на основе коллагена, трикальцийфосфата и пористой гидроксиапатитной керамикиComparative analysis of the efficiency of osteogenesis in peri-implant defect using scaffolds based on collagen, tricalcium phosphate and porous hydroxyapatite ceramicshttps://orcid.org/0000-0002-7450-3757РубниковичС. П.RubnikovichS. P.

Рубникович Сергей Петрович – член-корреспондент, д-р мед. наук, профессор, ректор.

Пр. Дзержинского, 83, 220116, Минск

Sergey P. Rubnikovich – Corresponding Member, D. Sc. (Med.), Professor, Rector.

83, Dzerzhinski Ave., 220116, Minsk

rubnikovich@mail.ruhttps://orcid.org/0000-0002-4924-5792СиракС. В.SirakS. V.

Сирак Сергей Владимирович – д-р мед. наук, профессор, заведующий кафедрой.

Ул. Мира, 310, 355017, Ставрополь

Sergey V. Sirak – D. Sc. (Med.), Professor, Head of the Department.

310, Mira Str., 355017, Stavropol

sergejsirak@yandex.ruhttps://orcid.org/0000-0003-0917-7972ДенисоваЮ. Л.DenisovaYu. L.

Денисова Юлия Леонидовна – д-р мед. наук, профессор.

Пр. Дзержинского, 83, 220116, Минск

Yuliya L. Denisova – D. Sc. (Med.), Professor.

83, Dzerzhinski Ave., 220116, Minsk

denisova-yul@mail.ruhttps://orcid.org/0000-0001-7004-3581ПериковаМ. Г.PerikovaM. G.

Перикова Мария Григорьевна – канд. мед. наук, доцент.

Ул. Мира, 310, 355017, Ставрополь

Maria G. Perikova – Ph. D. (Med.), Associate Professor.

310, Mira Str., 355017, Stavropol

https://orcid.org/0009-0000-5738-5501ЛеневВ. Н.LenevV. N.

Ленев Вадим Николаевич – канд. мед. наук, доцент.

Ул. Мира, 310, 355017, Ставрополь

Vadim N. Lenev ‒ Ph. D. (Med.), Associate Professor.

310, Mira Str., 355017, Stavropol

https://orcid.org/0000-0002-0573-7242БыковаН. И.BykovaN. I.

Быкова Наталья Ильинична – д-р мед. наук, доцент.

Ул. Седина, 4, 350063, Краснодар

Nataliya I. Bykova ‒ D. Sс. (Med.), Associate Professor.

4, Sedin Str., 350063, Krasnodar

nataliastom86@mail.ruhttps://orcid.org/0000-0001-8823-1409АрутюновА. В.ArutyunovA. V.

Арутюнов Арменак Валерьевич – д-р мед. наук, доцент, заведующий кафедрой.

Ул. Седина, 4, 350063, Краснодар

Armenak V. Arutyunov ‒ D. Sс. (Med.), Associate Professor, Head of the Department.

4, Sedin Str., 350063, Krasnodar

armenak@mail.ruhttps://orcid.org/0009-0004-8520-1246ШовгеновВ. Б.ShovgenovV. B.

Шовгенов Вячеслав Борисович – аспирант.

Ул. Седина, 4, 350063, Краснодар

Vyacheslav B. Shovgenov ‒ Postgraduate student.

4, Sedin Str., 350063, Krasnodar

Белорусский государственный медицинский университетBelarusian State MedicalСтавропольский государственный медицинский университетStavropol State Medical UniversityКубанский государственный медицинский университетKuban State Medical University202528052025222119133Copyright © Рубникович С.П., Сирак С.В., Денисова Ю.Л., Перикова М.Г., Ленев В.Н., Быкова Н.И., Арутюнов А.В., Шовгенов В.Б., 20252025Рубникович С.П., Сирак С.В., Денисова Ю.Л., Перикова М.Г., Ленев В.Н., Быкова Н.И., Арутюнов А.В., Шовгенов В.Б.Rubnikovich S.P., Sirak S.V., Denisova Y.L., Perikova M.G., Lenev V.N., Bykova N.I., Arutyunov A.V., Shovgenov V.B.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/1029

Для оценки эффективности остеогенеза в периимплантатном дефекте при использовании различных матриц для скаффолда необходимо выполнить точные информативные исследования – растровую электронную микроскопию (РЭМ) и спектральный анализ.

Цель исследования – изучить структуру и элементный состав костной ткани на поверхности дентальных имплантатов (ДИ) в периимплантатном дефекте после введения скаффолдов на основе губчато-кортикальной смеси аллогенного происхождения, коллагена и гидроксиапатита/β-трикальцийфосфата с предварительно подсаженными эктомезенхимальными клетками.

На полученной модели периимплантита у 12 экспериментальных однолетних овец cеверокавказской породы проводили хирургическое лечение периимплантатного дефекта с использованием скаффолдов на матрице из губчато-кортикальной смеси аллогенного происхождения (группа 1), коллагена (группа 2) и гидроксиапатита/ β-трикальцийфосфата (группа 3). Устанавливали ДИ с SA-поверхностью (подгруппа 1 в каждой группе) и СА-поверхностью (подгруппа 2 в каждой группе). Через 3 мес. после извлечения ДИ вместе с костным регенератом проводили РЭМ и спектральный анализ.

Костный регенерат вокруг ДИ в подгруппе 2 группы 2 значительно отличался по микроэлементному составу от других образцов. Содержание по весу кислорода (53,9 %), кальция (11,36 %), фосфора (7,04 %) соответствовало составу гидроксиапатита кальция, что свидетельствовало о высокой минерализации вновь образованной костной ткани.

Максимально эффективный остеогенез отмечен в подгруппе 2 группы 2, где матрицей для скаффолда выступал органический компонент – коллаген.

To evaluate the effectiveness of osteogenesis in the peri-implant defect using different scaffold matrices, it is necessary to perform accurate informative studies such as scanning electron microscopy and spectral analysis.

Objective: to study the structure and elemental composition of bone tissue on the surface of dental implants in the periimplant defect after the introduction of scaffolds based on a spongy-cortical mixture of allogeneic origin, collagen, and hydroxyapatite/β-tricalcium phosphate with pre-implanted ectomesenchymal cells.

On the obtained peri-implantitis model in 12 experimental one-year-old North Caucasian sheep, surgical treatment of the peri-implant defect was performed using scaffolds on a matrix of spongy-cortical mixture of allogeneic origin (group 1), collagen (group 2), and hydroxyapatite/β-tricalcium phosphate (group 3). Dental implants with SA-surface (1 subgroup in each group) and CA-surface (2 subgroup in each group) were installed. Scanning electron microscopy and spectral analysis were performed 3 months after extraction of dental implants together with bone regenerate.

Microelement composition of bone regenerate around dental implants of group 2 of subgroup 2 differed significantly from the composition of other samples. Content by weight of oxygen (53.9 %), calcium (11.36 %), phosphorus (7.04 %) corresponds to the composition of calcium hydroxyapatite, which indicates high mineralization of newly formed bone tissue.

The most effective osteogenesis was noted in the subgroup 2 of group 2, where the organic component – collagen – acted as a matrix for the scaffold.

β-трикальцийфосфатостеогенезкостная тканьпериимплантатный дефектрепаративная регенерациядентальный имплантатскаффолдэлектронная микроскопияспектрометрияβ-tricalcium phosphateosteogenesisbone tissueperi-implant defectreparative regenerationdental implantscaffoldelectron microscopyspectrometryReferencesEffect of a synthetic hydroxyapatite-based bone grafting material compared to established bone substitute materials on regeneration of critical-size bone defects in the ovine scapula / J. Wüster, N. Neckel, F. Sterzik [et al.] // Regenerative Biomaterials. – 2024. – Vol. 11. – P. rbae041. https://doi.org/10.1093/rb/rbae041Wüster J., Neckel N., Sterzik F., Xiang-Tischhauser L., Barnewitz D., Genzel A. [et al.]. Effect of a synthetic hydroxyapatite-based bone grafting material compared to established bone substitute materials on regeneration of criticalsize bone defects in the ovine scapula. Regenerative Biomaterials, 2024, vol. 11, p. rbae041. https://doi.org/10.1093/rb/rbae041Regeneration of alveolar ridge defects. Consensus report of group 4 of the 15th European workshop on periodontology on bone regeneration / S. Jepsen, F. Schwarz, L. Cordaro [et al.] // Journal of Clinical Periodontology. – 2019. – Vol. 21, suppl. 21. – P. 277–286. https://doi.org/10.1111/jcpe.13121Jepsen S., Schwarz F., Cordaro L., Derks J., Hämmerle C. H. F., Heitz-Mayfield L. J. [et al.]. Regeneration of alveolar ridge defects. Consensus report of group 4 of the 15th European workshop on periodontology on bone regeneration. Journal of Clinical Periodontology, 2019, vol. 21, suppl. 21, pp. 277–286. https://doi.org/10.1111/jcpe.13121Ridge expansion alone or in combination with guided bone regeneration to facilitate implant placement in narrow alveolar ridges: a retrospective study / Y. L. Tang, J. Yuan, Y. L. Song [et al.] // Clinical Oral Implants Research. – 2015. – Vol. 26, N 2. – P. 204–211. https://doi.org/10.1111/clr.12317Tang Y.-L., Yuan J., Song Y.-L., Ma W., Chao X., Li D.-H. Ridge expansion alone or in combination with guided bone regeneration to facilitate implant placement in narrow alveolar ridges: a retrospective study. Clinical Oral Implants Research, 2015, vol. 26, no. 2, pp. 204–211. https://doi.org/10.1111/clr.12317Рубникович, С. П. Регенеративные стоматологические технологии в комплексной хирургической и ортопедической реабилитации пациентов с дефектами зубных рядов / С. П. Рубникович, И. С. Хомич // Стоматолог. – 2020. – № 2. – C. 38–50.Rubnikovich S. P., Khomich I. S. Regenerative dental technologies in complex surgical and orthopedic rehabilitation of patients with dental defects. Stomatolog [Dentist], 2020, no. 2, pp. 38–50 (in Russian).Autograft, allograft, and bone graft substitutes: clinical evidence and Indications for use in the setting of orthopaedic trauma surgery / P. Baldwin, D. J. Li, D. A. Auston [et al.] // Journal of Orthopaedic Trauma. – 2019. – Vol. 33, N 4. – P. 203–213. https://doi.org/10.1097/BOT.0000000000001420Baldwin P., Li D. J., Auston D. A., Mir H. S., Yoon R. S., Koval K. J. Autograft, allograft, and bone graft substitutes: clinical evidence and Indications for use in the setting of orthopaedic trauma surgery. Journal of Orthopaedic Trauma, 2019, vol. 33, no. 4, pp. 203–213. https://doi.org/10.1097/BOT.0000000000001420Репаративная регенерация тканей пародонта – результаты экспериментального исследования / Е. В. Щетинин, С. В. Сирак, Л. А. Григорьянц [и др.] // Медицинский вестник Северного Кавказа. – 2015. – T. 4, № 10. – C. 411–415. https://doi.org/10.14300/mnnc.2015.10100Shchetinin E. V., Sirak S. V., Grigor’yants L. A., Vafiadi M. Yu., Dilekova O. V., Petrosyan G. G., Parazyan L. A., Gatilo Yu. Yu., Adamchik A. A. Reparative regeneration of periodontal tissues – results of an experimental study. Meditsinskii vestnik Severnogo Kavkaza [Medical Bulletin of the North Caucasus], 2015, vol. 4, no. 10, pp. 411–415 (in Russian). https://doi.org/10.14300/mnnc.2015.10100Rubnikovich, S. P. Morphological changes in bone tissue around dental implants after low-intensity ultrasound applications / S. P. Rubnikovich, I. S. Khomich, Yu. L. Denisova // Весцi Нацыянальнай акадэмii навук Беларусi. Серыя медыцынских навук. – 2020. – Т. 17, № 1. – С. 20–27.Rubnikovich S. P., Khomich I. S. Denisova Yu. L. Morphological changes in bone tissue around dental implants after low-intensity ultrasound applications. Vestsi Natsyyanal’nai akademii navuk Belarusi. Serуya medуtsynskikh navuk = Proceedings of the National Academy of Sciences of Belarus. Medical series, 2020, vol. 17, no. 1, pp. 20–27.Shang, L. Immunomodulatory properties: the accelerant of hydroxyapatite-based materials for bone regeneration / L. Shang, J. Shao, S. Ge // Tissue Engineering, Part C: Methods. – 2022. – Vol. 28, N 8. – Р. 377–392. https://doi.org/10.1089ten.TEC.2022.00111112Shang L., Shao J., Ge S. Immunomodulatory properties: the accelerant of hydroxyapatite-based materials for bone regeneration. Tissue Engineering, Part C: Methods, 2022, vol. 28, no. 8, pp. 377–392. https://doi.org/10.1089/ten.TEC.2022.00111112Arcos, D. Substituted hydroxyapatite coatings of bone implants / D. Arcos, M. Vallet-Regí // Journal of Materials Chemistry B. – 2020. – Vol. 8, N 9. – P. 1781–1800. https://doi.org/10.1039/c9tb02710fArcos D., Vallet-Regí M. Substituted hydroxyapatite coatings of bone implants. Journal of Materials Chemistry B, 2020, vol. 8, no. 9, pp. 1781–1800. https://doi.org/10.1039/c9tb02710fClinical, radiographic, and histological analyses after transplantation of crest-related palatal-derived ectomesenchymal stem cells (paldscs) for improving vertical alveolar bone augmentation in critical size alveolar defects / W. D. Grimm, W. A. Arnold, S. W. Sirak [et al.] // Journal of Clinical Periodontology. – 2015. – Vol. 42, N S17, poster P0986. – P. 366.Grimm W. D., Arnold W. A., Sirak S. W., Vukovich M. A., Videra D., Giesenhagen B. Clinical, radiographic, and histological analyses after transplantation of crest-related palatal-derived ectomesenchymal stem cells (paldscs) for improving vertical alveolar bone augmentation in critical size alveolar defects. Journal of Clinical Periodontology, 2015, vol. 42, no. S17, poster P0986, p. 366.Physicochemical characterization of porcine bone-derived grafting material and comparison with bovine xenografts for dental applications / J. H. Lee, G. S. Yi, J. W. Lee, D. J. Kim // Journal of Periodontal and Implant Science. – 2017. – Vol. 47, N 6. – P. 388–401. https://doi.org/10.5051/jpis.2017.47.6.388Lee J. H., Yi G. S., Lee J. W., Kim D. J. Physicochemical characterization of porcine bone-derived grafting material and comparison with bovine xenografts for dental applications. Journal of Periodontal and Implant Science, 2017, vol. 47, no. 6, pp. 388–401. https://doi.org/10.5051/jpis.2017.47.6.388Wickramasinghe, M. L. A novel classification of bone graft materials / M. L. Wickramasinghe, G. J. Dias, K. M. G. P. Premadasa // Journal of Biomedical Materials Research. Part B, Applied Biomaterials. – 2022. – Vol. 110, N 7. – P. 1724–1749. https://doi.org/10.1002/jbm.b.35029Wickramasinghe M. L., Dias G. J., Premadasa K. M. G. P. A novel classification of bone graft materials. Journal of Biomedical Materials Research. Part B, Applied Biomaterials, 2022, vol. 110, no. 7, pp. 1724–1749. https://doi.org/10.1002/jbm.b.35029Owen, G. Hydoxyapatite/beta-tricalcium phosphate biphasic ceramics as regenerative material for the repair of complex bone defects / G. Owen, M. Dard, H. Larjava // Journal of Biomedical Materials Research. Part B, Applied Biomaterials. – 2018. – Vol. 106, N 6. – P. 2493–2512. https://doi.org/10.1002/jbm.b.34049Owen G., Dard M., Larjava H. Hydoxyapatite/beta-tricalcium phosphate biphasic ceramics as regenerative material for the repair of complex bone defects. Journal of Biomedical Materials Research. Part B, Applied Biomaterials, 2018, vol. 106, no. 6, pp. 2493–2512. https://doi.org/10.1002/jbm.b.34049Bikuna-Izagirre, M. Gelatin blends enhance performance of electrospun polymeric scaffolds in comparison to coating protocols / M. Bikuna-Izagirre, J. Aldazabal, J. Paredes // Polymers. – 2022. – Vol. 14, N 7. – Art. 1311. https://doi.org/10.3390/polym14071311Bikuna-Izagirre M., Aldazabal J., Paredes J. Gelatin blends enhance performance of electrospun polymeric scaffolds in comparison to coating protocols. Polymers, 2022, vol. 14, no. 7, art. 1311. https://doi.org/10.3390/polym14071311Использование препарата Цифран СТ в хирургической стоматологии для лечения и профилактики послеоперационных воспалительных осложнений / Л. А. Григорьянц, Л. Н. Герчиков, В. А. Бадалян [и др.] // Стоматология для всех. – 2006. – № 2. – С. 14–16.Grigor’yants L. A., Gerchikov L. N., Badalyan V. A., Sirak S. V., Grigor’yants A. G. Use of the drug Tsifran ST in surgical dentistry for the treatment and prevention of postoperative inflammatory complications. Stomatologiya dlya vsekh [Dentistry for all], 2006, no. 2, pp. 14–16 (in Russian).Three-dimensional printing of a β-tricalcium phosphate scaffold with dual bioactivities for bone repair / M. Duan, S. Ma, C. Song [et al.] // Ceramics International. – 2021. – Vol. 47, N 4. – P. 4775–4782. https://doi.org/10.1016/j.ceramint.2020.10.047Duan M., Ma S., Song C., Li J., Qian M. Three-dimensional printing of a β-tricalcium phosphate scaffold with dual bioactivities for bone repair. Ceramics International, 2021, vol. 47, no. 4, pp. 4775–4782. https://doi.org/10.1016/j.ceramint.2020.10.047Gelatin-polycaprolactone-nanohydroxyapatite electrospun nanocomposite scaffold for bone tissue engineering / S. Gautam, C. Sharma, S. D. Purohit [et al.] // Materials Science and Engineering. C, Materials for biological applications. – 2021. – Vol. 119. – Art. 111588. https://doi.org/10.1016/j.msec.2020.111588Gautam S., Sharma C., Purohit S. D., Singh H., Dinda A. K., Potdar P. D., Chou C. F., Mishra N. C. Gelatin-polycaprolactone-nanohydroxyapatite electrospun nanocomposite scaffold for bone tissue engineering. Materials Science and Engineering. C, Materials for biological applications, 2021, vol. 119, art. 111588. https://doi.org/10.1016/j.msec.2020.111588A tissue engineered 3D printed calcium alkali phosphate bioceramic bone graft enables vascularization and regeneration of critical-size discontinuity bony defects in vivo / C. Knabe, M. Stiller, M. Kampschulte [et al.] // Frontiers in Bioengineering and Biotechnology. – 2023. – Vol. 11. – Art. 1221314. https://doi.org/10.3389/fbioe.2023.1221314Knabe C., Stiller M., Kampschulte M., Wilbig J., Peleska B., Günster J. [et al.]. A tissue engineered 3D printed calcium alkali phosphate bioceramic bone graft enables vascularization and regeneration of critical-size discontinuity bony defects in vivo. Frontiers in Bioengineering and Biotechnology, 2023, vol. 11, art. 1221314. https://doi.org/10.3389/fbioe.2023.1221314Mechanically stable β-TCP structural hybrid scaffolds for potential bone replacement / M. Ahlhelm, S. H. Latorre, H. O. Mayr [et al.] // Journal of Composites Science. – 2021. – Vol. 5, N 10. – Art. 281. https://doi.org/10.3390/jcs5100281Ahlhelm M., Latorre S. H., Mayr H. O., Storch C., Freytag C., Werner D., Schwarzer-Fischer E., Seidenstücker M. Mechanically stable β-TCP structural hybrid scaffolds for potential bone replacement. Journal of Composites Science, 2021, vol. 5, no. 10, art. 281. https://doi.org/10.3390/jcs51002813D printed poly (Caprolactone)/Hydroxyapatite scaffolds for bone tissue engineering: A comparative study on composite preparation by melt blending or solvent casting techniques and influence of bioceramic content on scaffold properties / S. Biscaia, M. V. Branquinho, R. D. Alvites [et al.] // International Journal of Molecular Sciences. – 2022. – Vol. 23, N 4. – Art. 2318. https://doi.org/10.3390/ijms23042318Biscaia S., Branquinho M. V., Alvites R. D., Fonseca R., Sousa A. C., Pedrosa S. S. [et al.]. 3D printed poly (Caprolactone)/Hydroxyapatite scaffolds for bone tissue engineering: A comparative study on composite preparation by melt blending or solvent casting techniques and influence of bioceramic content on scaffold properties. International Journal of Molecular Sciences, 2022, vol. 23, no. 4, art. 2318. https://doi.org/10.3390/ijms23042318Advances on bone substitutes through 3D bioprinting / T. Genova, I. Roato, M. Carossa [et al.] // International Journal of Molecular Sciences. – 2020. – Vol. 21, N 19. – Art. 7012. https://doi.org/10.3390/ijms21197012Genova T., Roato I., Carossa M., Motta C., Cavagnetto D., Mussano F. Advances on bone substitutes through 3D bioprinting. International Journal of Molecular Sciences, 2020, vol. 21, no. 19, art. 7012. https://doi.org/10.3390/ijms21197012Characterisation of bone regeneration in 3D printed ductile PCL/PEG/hydroxyapatite scaffolds with high ceramic microparticle concentrations / C. Cao, P. Huang, A. Prasopthum [et al.] // Biomaterials Science. – 2022. – Vol. 10, N 1. – P. 138–152. https://doi.org/10.1039/d1bm01645hCao C., Huang P., Prasopthum A., Parsons A. J., Ai F., Yang J. Characterisation of bone regeneration in 3D printed ductile PCL/PEG/hydroxyapatite scaffolds with high ceramic microparticle concentrations. Biomaterials Science, 2022, vol. 10, no. 1, pp. 138–152. https://doi.org/10.1039/d1bm01645hInfluence of 3D printing parameters on the mechanical stability of PCL scaffolds and the proliferation behavior of bone cells / F. Huber, D. Vollmer, J. Vinke [et al.] // Materials. – 2022. – Vol. 15, N 6. – Art. 2091. https://doi.org/10.3390/ma15062091Huber F., Vollmer D., Vinke J., Riedel B., Zankovic S., Schmal H., Seidenstuecker M. Influence of 3D printing parameters on the mechanical stability of PCL scaffolds and the proliferation behavior of bone cells. Materials, 2022, vol. 15, no. 6, art. 2091. https://doi.org/10.3390/ma15062091Engineering 3D-printed core-shell hydrogel scaffolds reinforced with hybrid hydroxyapatite/polycaprolactone nanoparticles for in vivo bone regeneration / S. E. El-Habashy, A. H. El-Kamel, M. M. Essawy [et al.] // Biomaterials Science. – 2021. – Vol. 9, N 11. – P. 4019–4039. https://doi.org/10.1039/d1bm00062dEl-Habashy S. E., El-Kamel A. H., Essawy M. M., Abdelfattah E. Z. A., Eltaher H. M. Engineering 3D-printed coreshell hydrogel scaffolds reinforced with hybrid hydroxyapatite/polycaprolactone nanoparticles for in vivo bone regeneration. Biomaterials Science, 2021, vol. 9, no. 11, pp. 4019–4039. https://doi.org/10.1039/d1bm00062dРегенеративные клеточные технологии в лечении рецессии десны / С. П. Рубникович, Ю. Л. Денисова, Т. Э. Владимирская [и др.] // Современные технологии в медицине. – 2018. – T. 10, № 4. – C. 94–104.Rubnikovich S. P., Denisova Yu. L., Vladimirskaya T. E., Andreeva V. A., Kvacheva Z. B., Panasenkova G. Yu., Volotovskii I. D. Regenerative cell technologies for gingival recession treatment. Sovremennye tekhnologii v meditsine = Modern technologies in medicine, 2018, vol. 10, vol. 4, pp. 94–104 (in Russian).Recent advance in surface modification for regulating cell adhesion and behaviors / S. Cai, C. Wu, W. Yang [et al.] // Nanotechnology Reviews. – 2020. – Vol. 1, N 9. – P. 971–989. https://doi.org/10.1515/ntrev-2020-0076Cai S., Wu C., Yang W., Liang W., Yu H., Liu L. Recent advance in surface modification for regulating cell adhesion and behaviors. Nanotechnology Reviews, 2020, vol. 1, no. 9, pp. 971–989. https://doi.org/10.1515/ntrev-2020-0076Bernatskiy, B. S. A novel approach for implant rehabilitation combined with immediate bone and soft-tissue augmentation in a compromised socket-A B2S approach: case report with a 2-year follow-up / B. S. Bernatskiy, A. Puišys // Case Reports in Dentistry. – 2023. – Vol. 2023. – Art. 1376588. https://doi.org/10.1155/2023/1376588Bernatskiy B. S., Puišys A. A novel approach for implant rehabilitation combined with immediate bone and softtissue augmentation in a compromised socket-A B2S approach: case report with a 2-year follow-up. Case Reports in Dentistry, 2023, vol. 2023, art. 1376588. https://doi.org/10.1155/2023/1376588Efficacy of bone-substitute materials use in immediate dental implant placement: a systematic review and metaanalysis / J. Zaki, N. Yusuf, A. El-Khadem [et al.] // Clinical Implant Dentistry and Related Research. – 2021. – Vol. 23, N 4. – P. 506–519. https://doi.org/10.1111/cid.13014Zaki J., Yusuf N., El-Khadem A., Scholten R. J. P. M., Jenniskens K. Efficacy of bone-substitute materials use in immediate dental implant placement: a systematic review and meta-analysis. Clinical Implant Dentistry and Related Research, 2021, vol. 23, no. 4, pp. 506–519. https://doi.org/10.1111/cid.13014Sala, Y. M. Clinical outcomes of maxillary sinus floor perforation by dental implants and sinus membrane perforation during sinus augmentation: a systematic review and meta-analysis / Y. M. Sala, H. Lu, B. R. Chrcanovic // Journal of Clinical Medicine. – 2024. – Vol. 13, N 5. – Art. 1253. https://doi.org/10.3390/jcm13051253Sala Y. M., Lu H., Chrcanovic B. R. Clinical outcomes of maxillary sinus floor perforation by dental implants and sinus membrane perforation during sinus augmentation: a systematic review and meta-analysis. Journal of Clinical Medicine, 2024, vol. 13, no. 5, art. 1253. https://doi.org/10.3390/jcm13051253Efficacy of lateral bone augmentation performed simultaneously with dental implant placement: a systematic review and meta-analysis / D. S. Thoma, S. P. Bienz, E. Figuero [et al.] // Journal of Clinical Periodontology. – 2019. – Vol. 46, N 21. – P. 257–276. https://doi.org/10.1111/jcpe.13050Thoma D. S., Bienz S. P., Figuero E., Jung R. E., Sanz-Martín I. Efficacy of lateral bone augmentation performed simultaneously with dental implant placement: a systematic review and meta-analysis. Journal of Clinical Periodontology, 2019, vol. 46, no. 21, pp. 257–276. https://doi.org/10.1111/jcpe.13050Commercialization and regulation of regenerative medicine products: Promises, advances and challenges / N. Beheshtizadeh, M. Gharibshahian, Z. Pazhouhnia [et al.] // Biomedicine and Pharmacotherapy. – 2022. – Vol. 153. – Art. 113431. https://doi.org/10.1016/j.biopha.2022.113431Beheshtizadeh N., Gharibshahian M., Pazhouhnia Z., Rostami M., Zangi A. R., Maleki R. [et al.]. Commercialization and regulation of regenerative medicine products: Promises, advances and challenges. Biomedicine and Pharmacotherapy, 2022, vol. 153, art. 113431. https://doi.org/10.1016/j.biopha.2022.113431In situ magnesium phosphate/polycaprolactone 3D-printed scaffold induce bone regeneration in rabbit maxillofacial bone defect model / B. Lei, X. Gao, R. Zhang [et al.] // Materials and Design. – 2022. – Vol. 215. – Art. 110477. https://doi.org/10.1016/j.matdes.2022.110477Lei B., Gao X., Zhang R., Yi X., Zhou, Q. In situ magnesium phosphate/polycaprolactone 3D-printed scaffold induce bone regeneration in rabbit maxillofacial bone defect model. Materials and Design, 2022, vol. 215, art. 110477. https://doi.org/10.1016/j.matdes.2022.110477Effect of zinc-doped hydroxyapatite/graphene nanocomposite on the physicochemical properties and osteogenesis differentiation of 3D-printed polycaprolactone scaffolds for bone tissue engineering / H. Maleki-Ghaleh, M. Hossein Siadati, A. Fallah [et al.] // Chemical Engineering Journal. – 2021. – Vol. 426. – Art. 131321. https://doi.org/10.1016/j.cej.2021.131321Maleki-Ghaleh H., Hossein Siadati M., Fallah A., Zarrabi A., Afghah F., Koc B. [et al.]. Effect of zinc-doped hydroxyapatite/graphene nanocomposite on the physicochemical properties and osteogenesis differentiation of 3D-printed polycaprolactone scaffolds for bone tissue engineering. Chemical Engineering Journal, 2021, vol. 426, art. 131321. https://doi.org/10.1016/j.cej.2021.131321Surface potential and roughness controlled cell adhesion and collagen formation in electrospun PCL fibers for bone regeneration / S. Metwally, S. Ferraris, S. Spriano [et al.] // Materials and Design. – 2020. – Vol. 194. – Art. 108915. https://doi.org/10.1016/j.matdes.2020.108915Metwally S., Ferraris S., Spriano S., Krysiak Z. J., Kaniuk Ł., Marzec M. M. [et al.]. Surface potential and roughness controlled cell adhesion and collagen formation in electrospun PCL fibers for bone regeneration. Materials and Design, 2020, vol. 194, art. 108915. https://doi.org/10.1016/j.matdes.2020.108915Youssef, M. A. Diagnostic reliability and accuracy of the hydraulic contrast lift protocol in the radiographic detection of sinus lift and perforation: ex vivo randomized split-mouth study in an ovine model / M. A. Youssef, N. von Krockow, J. A. Pfaff // Biodiversity Data Journal. – 2024. – Vol. 10, N 1. – Art. 6. https://doi.org/10.1038/s41405-024-00188-6Youssef M. A., Von Krockow N., Pfaff J. A. Diagnostic reliability and accuracy of the hydraulic contrast lift protocol in the radiographic detection of sinus lift and perforation: ex vivo randomized split-mouth study in an ovine model. Biodiversity Data Journal, 2024, vol. 10, no. 1, art. 6. https://doi.org/10.1038/s41405-024-00188-6Effect of the surface morphology of silk fibroin scaffolds for bone regeneration / U. K. Bhawal, R. Uchida, N. Kuboyama [et al.] // Journal of Biomedical Materials Research. – 2016. – Vol. 27, N 4. – P. 413–424. https://doi.org/10.3233/BME-161595Bhawal U. K., Uchida R., Kuboyama N., Asakura T., Hiratsuka K., Nishiyama N. Effect of the surface morphology of silk fibroin scaffolds for bone regeneration. Journal of Biomedical Materials Research, 2016, vol. 27, no. 4, pp. 413–424. https://doi.org/10.3233/BME-161595

The authors declare that there are no conflicts of interest present.