vestimИзвестия Национальной академии наук Беларуси. Серия медицинских наукProceedings of the National Academy of Sciences of Belarus, Medical series1814-60232524-2350The Republican Unitary Enterprise Publishing House "Belaruskaya Navuka"10.29235/1814-6023-2025-22-1-57-64vestim-1015Research ArticleКЛИНИЧЕСКАЯ И ЭКСПЕРИМЕНТАЛЬНАЯ МЕДИЦИНАCLINICAL AND EXPERIMENTAL MEDICINEЭффект аскорбиновой кислоты на получение преддифференцированных в хондрогенном направлении мезенхимальных стволовых/стромальных клетокEffect of ascorbic acid on obtaining chondrogenic pre-differentiated mesenchymal stem/stromal cellshttps://orcid.org/0009-0006-9593-7067ЖерносеченкоА. А.ZhernasechankaH. A.

Жерносеченко Анна Александровна – канд. биол. наук, вед. науч. сотрудник

ул. Фрунзенская, 43, 223053, Минский р-н,  д. Боровляны

Hanna A. Zhernasechanka – Ph. D. (Biol.), Leading Researcher

43, Frunzenskaya Str., v. Borovliany, 223053, Minsk Region

sapphire.anna@gmail.comhttps://orcid.org/0000-0001-5196-8554ИсайкинаЯ. И.IsaikinaYa. I.

Исайкина Янина Ивановна – канд. биол. наук, заведующий лабораторией

ул. Фрунзенская, 43, 223053, Минский р-н,  д. Боровляны

Yanina I. Isaikina – Ph. D. (Biol.), Head of the Laboratory

43, Frunzenskaya Str., v. Borovliany, 223053, Minsk Region

yaninai@mail.ruРеспубликанский научно-практический центр детской онкологии, гематологии и иммунологииRepublican Research Center for Pediatric Oncology, Hematology and Immunology2025280220252215764Copyright © Жерносеченко А.А., Исайкина Я.И., 20252025Жерносеченко А.А., Исайкина Я.И.Zhernasechanka H.A., Isaikina Y.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestimed.belnauka.by/jour/article/view/1015

Мезенхимальные стволовые/стромальные клетки (МСК) обладают уникальными функциональными свойствами, в частности способностью дифференцироваться в хондрогенном направлении. Благодаря этому данная популяция клеток широко востребована при репарации хрящевой ткани. Различные комбинации ростовых факторов не только способствуют экспрессии генов-маркеров гиалинового хряща, но и индуцируют маркеры, ассоциированные с гипертрофией. Есть данные, что аскорбиновая кислота может стимулировать хондрогенную дифференцировку МСК за счет увеличения транскрипции генов коллагенов Сol2. В связи с этим изучено влияние аскорбиновой кислоты при ее добавлении в дифференцировочную среду к ранее разработанному коктейлю цитокинов с целью получения гиалиноподобного матрикса. Ввиду спорного времени индукции процесса хондрогенеза в МСК сделано предположение о возможности сокращения срока индукции хондрогенной преддифференцировки МСК.

На основе проведенных исследований подтверждено положительное влияние аскорбиновой кислоты на экспрессию гена Col2, что свидетельствует об образовании гиалиноподобной хрящевой ткани при хондрогенной дифференцировке МСК. Анализ экспрессии генов-маркеров хондрогенной дифференцировки МСК и синтеза компонентов внеклеточного матрикса на 4-е и 7-е сутки показал возможность сокращения срока преддифференцировки до 4 сут.

Mesenchymal stem/stromal cells (MSCs) have unique functional properties, one of which is the chondrogenic differentiation. This population of cells is being used in clinical practice for repairing cartilage tissue. Various combinations of growth factors promote the expression of markers of hyaline cartilage, but also induce hypertrophy’s markers. There  is evidence that ascorbic acid can promote chondrogenic differentiation of MSCs by increasing the transcription of Col2, so we have studied when adding ascorbic acid to the differentiation medium to the previously developed cytokine cocktail in order to obtain a hyaline-like matrix. There is a different time of induction of chondrogenesis in MSCs, so we assumed to reduce the induction period of chondrogenic pre-differentiation of MSCs. Based on our studies we confirmed a positive effect of ascorbic acid on the expression of Col2, which indicates the possibility of obtaining strong and stable chondrogenic differentiation  of MSCs. The analysis of the expression of genes-markers of chondrogenic differentiation of MSCs and the synthesis  of extracellular matrix components on the 4th and 7th days showed a possibility of reducing the pre-differentiation period  to 4 days.

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The authors declare that there are no conflicts of interest present.